Biomedical

Recent Advances in Metalloproteomics

Institution: rnfinity

Email: info@rnfinity.com

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James P. C. Coverdale,

James P. C. Coverdale

Institution: School of Pharmacy, Institute of Clinical Sciences, University of Birmingham, Edgbaston B15 2TT, UK

Email:

Sirilata Polepalli,

Sirilata Polepalli

Institution: Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK

Email:

Marco A. Z. Arruda,

Marco A. Z. Arruda

Institution: Institute of Chemistry, Department of Analytical Chemistry, Universidade Estadual de Campinas, Campinas 13083-970, Brazil

Email:

Ana B. Santos da Silva,

Ana B. Santos da Silva

Institution: Institute of Chemistry, Department of Analytical Chemistry, Universidade Estadual de Campinas, Campinas 13083-970, Brazil

Email:

Alan J. Stewart,

Alan J. Stewart

Institution: School of Medicine, University of St. Andrews, St Andrews KY16 9TF, UK

Email:

Claudia A. Blindauer

Claudia A. Blindauer

Institution: Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK

Email:

Abstract

Interactions between proteins and metal ions and their complexes are important in many areas of the life sciences, including physiology, medicine, and toxicology. Despite the involvement of essential elements in all major processes necessary for sustaining life, metalloproteomes remain ill-defined. This is not only owing to the complexity of metalloproteomes, but also to the non-covalent character of the complexes that most essential metals form, which complicates analysis. Similar issues may also be encountered for some toxic metals. The review discusses recently developed approaches and current challenges for the study of interactions involving entire (sub-)proteomes with such labile metal ions. In the second part, transition metals from the fourth and fifth periods are examined, most of which are xenobiotic and also tend to form more stable and/or inert complexes. A large research area in this respect concerns metallodrug–protein interactions. Particular attention is paid to separation approaches, as these need to be adapted to the reactivity of the metal under consideration.

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2024-11-03

Doi: http://dx.doi.org/10.3390/biom14010104
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