Biomedical

Automated Patch Clamp for the Detection of Tetrodotoxin in Pufferfish Samples

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Mònica Campàs,

Mònica Campàs

IRTA, Marine and Continental Waters (AMiC) Programme, Ctra. Poble Nou del Delta, km. 5.5, 43540 La Ràpita, Spain


Jaume Reverté,

Jaume Reverté

IRTA, Marine and Continental Waters (AMiC) Programme, Ctra. Poble Nou del Delta, km. 5.5, 43540 La Ràpita, Spain


Àngels Tudó,

Àngels Tudó

Department of Basic Medical Sciences, Universitat Rovira i Virgili, C/Sant Llorenç 21, 43201 Reus, Spain


Mounira Alkassar,

Mounira Alkassar

IRTA, Marine and Continental Waters (AMiC) Programme, Ctra. Poble Nou del Delta, km. 5.5, 43540 La Ràpita, Spain


Jorge Diogène,

Jorge Diogène

IRTA, Marine and Continental Waters (AMiC) Programme, Ctra. Poble Nou del Delta, km. 5.5, 43540 La Ràpita, Spain


Francesc X. Sureda

Francesc X. Sureda

Department of Basic Medical Sciences, Universitat Rovira i Virgili, C/Sant Llorenç 21, 43201 Reus, Spain


  Peer Reviewed

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© attribution CC-BY

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2024-11-03

Doi: http://dx.doi.org/10.3390/md22040176
Related Subjects
Anatomy
Biochemistry
Epidemiology
Genetics
Neuroscience
Psychology
Oncology
Medicine
Musculoskeletal science
Pediatrics
Pathology
Pharmacology
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Abstract

Tetrodotoxin (TTX) is a marine toxin responsible for many intoxications around the world. Its presence in some pufferfish species and, as recently reported, in shellfish, poses a serious health concern. Although TTX is not routinely monitored, there is a need for fast, sensitive, reliable, and simple methods for its detection and quantification. In this work, we describe the use of an automated patch clamp (APC) system with Neuro-2a cells for the determination of TTX contents in pufferfish samples. The cells showed an IC50 of 6.4 nM for TTX and were not affected by the presence of muscle, skin, liver, and gonad tissues of a Sphoeroides pachygaster specimen (TTX-free) when analysed at 10 mg/mL. The LOD achieved with this technique was 0.05 mg TTX equiv./kg, which is far below the Japanese regulatory limit of 2 mg TTX equiv./kg. The APC system was applied to the analysis of extracts of a Lagocephalus sceleratus specimen, showing TTX contents that followed the trend of gonads > liver > skin > muscle. The APC system, providing an in vitro toxicological approach, offers the advantages of being sensitive, rapid, and reliable for the detection of TTX-like compounds in seafood.

Related Subjects
Anatomy
Biochemistry
Epidemiology
Genetics
Neuroscience
Psychology
Oncology
Medicine
Musculoskeletal science
Pediatrics
Pathology
Pharmacology
Physiology
Psychiatry
Primary care
Women and reproductive health
copyright icon

© attribution CC-BY

  • 0

rating
111 Views

Added on

2024-11-03

Doi: http://dx.doi.org/10.3390/md22040176
Related Subjects
Anatomy
Biochemistry
Epidemiology
Genetics
Neuroscience
Psychology
Oncology
Medicine
Musculoskeletal science
Pediatrics
Pathology
Pharmacology
Physiology
Psychiatry
Primary care
Women and reproductive health